CONTROL OF POLYMER PARTICLE SIZE USING POROUS GLASS MEMBRANE EMULSIFICATION A REVIEW

1.IntroductionPolymerparticleshavebeenusedinvariousfields.Besidestraditionalapplicationssuchaspaint,ink,andpaperindustries,muchattentionhasinrecentyearsbeenpaidtofineapplications,e.g.,carrierforenzyme(Omietal.,1997),separationmedia(Okubo&Hatorri,1993;Inomataetal.,1995;Ugelstadetal.,1992)forprotein,DNAandcell,andcarrierfordruginDrugDeliverySystem(DDS)(Paulettietal.,1997;Russell-Jones,2000).Controlofpolymerparticlesizeisespeciallyimportantinsuchfineapplications.Forexample,whenporouspartic…     

多约束膜结构截面优化及在MSC/Nastran上的程序实现

将准则法和数学规划法相结合，借助满应力准则将应力约束转化为动态尺寸约束，利用单位虚载荷法将位移约束转化为设计变量的显式表达式建立优化模型，然后用数学规划法求解；采用无量纲设计变量实现设计变量连接，对膜结构的厚度进行优化设计；根据对偶理论，应用对偶规划精确映射原问题，再按泰勒展式建立对偶问题的二阶近似。为了提高优化效率，采用射线步调整结构性态，运用粗选有效约束技术筛选约束，并采用主、被动变量循环确保收敛稳定。以MSC／Nastran软件作为结构分析的求解器，以MSC／Patran软件作为开发平台，完成了膜结构截面优化程序。对膜结构的单变位、多变位的结构优化问题进行了优化计算，并与MSC／Nastran优化模块的计算结果进行比较。算例结果表明程序的可靠性、高效性和稳定性以及理论算法的优越性。     

Surface strain-field determination of tympanic membrane using 3D-digital holographic interferometry

In order to increase the understanding of soft tissues mechanical properties, 3D Digital Holographic Interferometry (3D-DHI) was used to quantify the strain-field on a cat tympanic membrane (TM) surface. The experiments were carried out applying a constant sound-stimuli pressure of 90 dB SPL (0.632 Pa) on the TM at 1.2 kHz. The technique allows the accurate acquisition of the micro-displacement data along the x, y and z directions, which is a must for a full characterization of the tissue mechanical behavior under load, and for the calculation of the strain-field in situ. The displacements repeatability in z direction shows a standard deviation of 0.062 µm at 95% confidence level. In order to realize the full 3D characterization correctly the contour of the TM surface was measured employing the optically non-contact two-illumination positions contouring method. The x, y and z displacements combined with the TM contour data allow the evaluation its strain-field by spatially differentiating the u(m,n), v(m,n), and w(m,n) deformation components. The accurate and correct determination of the TM strain-field leads to describing its elasticity, which is an important parameter needed to improve ear biomechanics studies, audition processes and TM mobility in both experimental measurements and theoretical analysis of ear functionality and its modeling.     

Early Membrane Responses to Magnetic Particles are Predictors of Particle Uptake in Neural Stem Cells

Magnetic particles (MPs) offer several advantages for neural cell therapy, but limited particle uptake by neural cells is a barrier to translation. It is recently proved that tailoring particle physicochemical properties (by enhancing their iron content) dramatically improves uptake in neural stem cells (NSCs)—a major transplant population. High‐throughput screening of particles with varying physicochemical properties can therefore aid in identifying particles with optimal uptake features, but research is hampered by the lack of simple methodologies for studying neural cell membrane responses to nanoparticle platforms. A high‐resolution–high throughput method has been used to study early membrane responses of primary rodent NSCs to particles of variant magnetite loading, to attempt to correlate these responses with known particle internalization profiles. Membrane imaging is enhanced through sequential staining with osmium (O) and thiocarbohydrazide (T), a method termed OTOTO, combined with field‐emission scanning electron microscopy (FESEM). A five‐point classification system was used to systematically evaluate early MP‐induced membrane responses to particles possessing distinct physicochemical properties. Significantly different profiles of membrane activation were noted that correlate with particle uptake profiles. It is suggested that our method can serve as a valuable predictor of particle internalization in neural cells for diverse particle platforms.     

A comparison of the surface nanostructure from two different types of gram-negative cells: Escherichia coli and Rhodobacter sphaeroides

Bacteria have been studied using different microscopy methods for many years. Recently, the developments of high-speed atomic force microscopy have opened the doors to study bacteria in new ways due to the fact that it uses much less force on the sample while imaging. This makes the high-speed atomic force microscope an indispensable technique for imaging the surface of living bacterial cells because it allows for the high-resolution visualization of surface proteins in their natural condition without disrupting the cell or the activity of the proteins. Previous work examining living cells of Magnetospirillum magneticum AMB-1 demonstrated that the surface of these bacteria was covered with a net-like structure that is mainly composed of porin molecules. However, it was unclear whether or not this feature was unique to other living bacteria. In this study we used the high-speed atomic force microscope to examine the surface of living cells of Escherichia coli and Rhodobacter sphaeroides to compare their structure with that of M. magneticum. Our research clearly demonstrated that both of these types of cells have an outer surface that is covered in a network of nanometer-sized holes similar to M. magneticum. The diameter of the holes was 8.0 ± 1.5 nm for E. coli and 6.6 ± 1.1 nm for R. sphaeroides. The results in this paper confirm that this type of outer surface structure exists in other types of bacteria and it is not unique to Magnetospirillum.     

Boundary conditions for stokes flows near a porous membrane

A theoretical development is carried out to model the boundary conditions for Stokes flows near a porous membrane, which, in general, allows non-zero slip as well as normal flow at the surface. Two types of models are treated: an infinitesimally thin plate with a periodic array of circular apertures and a series of parallel slits. For Stokes flows, the mean normal flux and slip velocity are proportional to the pressure difference across the membrane and the average shear stress at the membrane, respectively. The appropriate proportionality constants which depend on the membrane geometry are calculated as functions of the porosity. An interesting feature of the results is that the slip at the membrane has, in general, a direction different from that of the applied shear for these models.     

大跨度脊谷式膜屋盖风载分布的实验研究

基于台州某风雨操场脊谷式张拉膜屋盖缩尺模型风洞试验的数据结果,选取典型测点,研究了屋面迎风前缘、过渡区及中轴区的平均风压和脉动风压系数的分布特性。同时,鉴于屋盖的不规则曲面造型,表面风压梯度变化较大,采用单一体型系数反映屋面风载已不能满足要求。文中在结合屋盖自身复杂体型和风压分布特征基础上,按各榀各边片将屋面划分为不同区域,对5个不利风向角下的区域体型系数进行统计分析,并给出各区域体型系数建议取值。最后,针对这类体型屋盖特点和风压分布特性,得出一些结论和建议,为进一步研究该类屋盖的风荷载特性和结构抗风设计提供了依据。     

Analysis of blank thickening in deep drawing processes using the theory of a Cosserat generalized membrane

This paper analyzes a transient, nonlinear deep drawing process where a circular blank of a rigid-plastic material is forced by a rigid circular punch to deform into a cylindrical cup. Attention is focused on the plastic flow beneath the blank-holder. Using the Cosserat theory of a generalized membrane it is possible to obtain analytical solutions which examine the following two major effects: (a) the importance of added “rim pressure” acting on the outer edge of the blank; and (b) the importance of a controlled moveable blank-holder to allow blank thickening during the drawing process. Guided by these analytical results, a new deep drawing machine was built to exploit these effects and increase the limit drawing ratio (LDR) of the drawing process. Specifically, the LDR (in one stroke) reached the value of 3.16 compared with the value of about 2.0 in the conventional process. Moreover, the analytical prediction of the punch force versus the punch stroke is in good agreement with the experimental data and with simulations using the computer code DYTRAN.     

含有序孔洞的多孔氧化铝薄膜的弹性模量测量

高纯铝箔在特定的溶液下经过电化学阳极氧化腐蚀，可在其表面生成一层多孔的非晶氧化铝层，孔大致呈六方密排，孔径分布均匀。此类薄膜具有规则的纳米级孔径，大的比表面积，可用在微纳滤方面和纳米材料组装方面。然而，对于此类薄膜力学性能的研究较少，在一定程度上限制其功能的开发和应用。为了获得此类多孔膜的弹性常数，本文用鼓膜法结合散斑干涉实验方法、单轴拉伸结合双光束干涉法和多普勒测振仪三种方法测量氧化铝多孔膜的弹性模量，得到的宏观弹性模量基本相同，并对三种方法的优缺点进行了比较，分析了多孔氧化铝膜与块状氧化铝材料或致密氧化铝膜力学性能的差并。